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   Shumaila Idrees on February 13, 2019 at 3:45 pm

   BT301 Assignment
   Solution Idea
   Role of RFLP in crime investigation:
   DNA typing technique primary identify specific genetic markers that are highly polymorphic within a population and have found great utility in forensic science. The establish DNA identification protocol, term RFLP, has been admitted a physical evidence in the investigation of crime such as assault, sexual assault and homicide. The limitation association with this procedure concerns the integrity of the genetic material. This study sought to evaluate human bone as a source material for DNA identification following exposure to common forensic field condition. Often, with the onset of decomposition and eventual disarticulation of a body, soft body, hair and teeth may not be recovered. Significance of this study lies in the fact that, within forensic arthaplology, human bone represents the most biological stable and is sometime all that remains after period of exposure. Genomic DNA was extracted from human bone following exposure to surface deposit, shallow burial and fresh water immersion. Sample were collected over three month time course and analyzed by spectrometry and agars gel electrophori’s as a well as RFLP analyze.
   RFLP: Analysis Technique and applications
   RFLP analysis technique involved in cutting a particular reign of DNA with known variability, with restriction enzyme then separating the DNA fragments by arose gel electrophoreses and determining the number of fragment and relative size .
   A restriction enzyme is an enzyme, a protein molecule the cut DNA at restriction site. In essence the DNA sample is broken up and digested by the restriction enzyme. The resulting fragment is separating according to their length and the pattern of fragment size will differ for each other individual tested.
   The full RFLP process requires probe labeling, DNA fragmentation, electrophoresis, blotting, hybridization, washing and autoradiography. The detected RFLP is visualized using X-film in autoradiography, where DNA fragment can be viewed and analyzed after they are separated from one another by electrophoresis
   Some of application of RFLP analysis includes:
   DNA fingerprinting:
   Forensic science may use RFLP amyls to identify suspect based on evidence sample collected at screen of crimes.
   Paternity:
   RFLP is also used in determination paternity or for tracing ancestry.
   Genetic diversity:
   The technique can be used in study evolution and migration of wildlife, studying breeding pattern in animal’s population and detection and diagnoses in certain disease.
   Result from human project to pretty much replace the need for RFLP. The project allowed for deterring the interior sequence of the DNA found in human cell, the human genome and for identification the entire gene in the human genome.